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Question

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希望语言流畅 有条理
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The dialyzed sample was subject to a column of Sephadex G-25 and eluted with 0.2 M acetic acid. Fraction of 4.5 ml each was collected on fraction collector at a flow rate of 48 ml/h.
The absorbance at 280 nm was measured to monitor the protein during chromatography separation. The protein content of water extract was measured by the method of Lowry et al. (1951), using bovine serum albumin as standard.
The homogeneity of pooled fractions were checked by SDS disc gel electrophoresis by applying 50 ml of denatured sample on the surface of the gel. Power supply was adjusted at 5 mA current per tube for 80 min. The gel was stained with Coomassie blue R 250 and destained with methanol: acetic acid: water as reported by Davis, Hames and Rickwood.
The cultures of bacteria grown overnight at 37°C were used for testing the antibacterial activity from different fractions separated on Sephadex G-25 and aqueous extract of I. oblongifolia leaves. The anti-bacterial activity was checked by seed plate method as reported by Rashed et al.. In this technique meat extract nutrient medium containing 1.5% agar was adjusted to pH 7.0, distributed in 40 ml quantity in screw capped bottles and sterilized. The bacterial culture was then added aseptically to the agar medium at 45°C, mixed well and poured immediately in sterilized petri-plates. After hardening, 6 mm diameter well were cut into agar and 100 ml of the I. oblongifolia leave extract and fractions were placed in these wells. The plates were incubated at 37°C and observations were made after 24–72 h. The zone inhibition produced by the plant fractions were compared with zone inhibition produced by commercial standard antibiotics disc such as Tarvid and Enaxbid.
Antifungal activity was tested against Aspergillus niger A25717, A. fumigatus A9381, A. fla6us A15197 and P. expansum CMI 39761. The diffusion plate method was used to test I. oblongifolia leave fractions with slight modification as reported by Terras et al. (1995). In this technique, 0.1 ml of the fungal spore suspension (grown for 3 days in 10 ml of nutrient Dextrose agar) was thoroughly mixed with 20 ml of melted Sabouraud dextrose agar and poured into sterilized petri plates. When the agar was set, 5 wells of 6 mm diameter were made on each of the seeded plate. These holes were filled with 100 ml of the testing sample. All these experiments were performed in duplicate. The petri plates were incubated at 28°C for 7–8 days. All the culture plates were examined after 24–96 h and the results are tabulated. The zone inhibition produced by the plant fractions were compared with zone produced by the standards (nystatin and griseofulvin 20 mg/ml).

Answer 1

该透析样本受一经Sephadex G - 25柱和0.2米醋酸洗脱。每个部位的4.5毫升搜集有关馏分收集在48毫升/小时流量
在280 nm处测定吸光度，监察色谱分离蛋白。水的提取蛋白质含量测定的劳里等人的方法。 （1951年），并以此作为标准的牛血清白蛋白。
汇集组分均匀性进行了检查用SDS凝胶电泳采用光盘对变性凝胶样品表面50毫升。电力供应在5毫安调整为80分钟，每管电流。凝胶与考马斯亮蓝R 250染色和destained与甲醇：乙酸：水由戴维斯报道，阿梅斯和里克伍德。
培养中生长的细菌在37℃过夜进行检测，从对经Sephadex G - 25分离不同组分的抗菌活性和水提取物对一oblongifolia用树叶。该抗菌活性检查种子平板法所报道拉希德等..这种技术肉类提取物营养琼脂培养基中15％调整为pH值7.0，分布在40毫升瓶螺杆上限数量和消毒。细菌培养，然后加入到无菌的琼脂在45℃中，混合好，倒在消毒的Petri板立即。硬化后，直径6毫米以及切成琼脂和100毫升的一oblongifolia离开提取和分数在这些油井上。板块孵育37 ° C和意见，是经过24-72小时该地带抑制植物组分产生了比较抗生素与商业标准，如Tarvid和Enaxbid光盘生产区的抑制作用。
抗真菌活性，对黑曲霉A25717测试，烟曲霉A9381，甲fla6us A15197和青霉海事39761。扩散板的方法来测试一oblongifolia留下轻微的修改分数由拉斯等报道。 （1995年）。这种技术，0.1真菌孢子悬浮生长在3天10毫升的营养葡萄糖琼脂（毫升）被彻底融化混合20毫升沙氏琼脂并灭菌培养皿倒。当琼脂集，5毫米直径分别为6对种子每盘了水井。这些洞充满了100毫升的测试样品。所有这些实验，一式两份。孵育的Petri板在28 ° C的7-8天。所有被检查的培养板后24-96小时，结果表列。该区域的抑制植物组分产生了与标准（生产区相比，制霉菌素和灰黄霉素20毫克/毫升）。

Answer 2

该透析样本受一经Sephadex G - 25柱和醋酸洗脱0.2米。每个部位的4.5毫升搜集有关部分收藏家在48毫升/小时流量
在280 nm处测定吸光度，监察色谱分离蛋白。水的提取蛋白质含量测定的劳里等人的方法。 （1951年），并以此作为标准的牛血清白蛋白。
汇集组分均匀性进行了检查用SDS凝胶电泳采用光盘对变性凝胶样品表面50毫升。电力供应在5毫安调整为80分钟，每管电流。凝胶与考马斯亮蓝R 250染色和destained与甲醇：乙酸：水由戴维斯报道，阿梅斯和里克伍德。
培养中生长的细菌在37 ° C一夜之间从被测试的Sephadex G - 25分离不同组分的抗菌活性和水提取物对一oblongifolia用树叶。该抗菌活性检查种子平板法所报道拉希德等..这种技术肉类提取物营养琼脂培养基中的1.5％调整为pH值7.0
，分布在40毫升瓶螺杆上限数量和消毒。当时的细菌培养无菌添加到在45 ° C琼脂培养基，混合好，倒在消毒的Petri板立即。硬化后，直径6毫米以及切成琼脂，100毫升的一oblongifolia离开提取和分数在这些油井上。该
板放置于37 ° C和意见，是经过24-72小时该区域的抑制植物组分产生了比较抗生素与商业标准，如Tarvid和Enaxbid光盘生产区的抑制作用。
抗真菌活性，对黑曲霉A25717测试，烟曲霉A9381，甲fla6us A15197和青霉海事39761。扩散板的方法来测试一oblongifolia留下轻微的修改分数由拉斯等报道。 （1995年）。这种技术，0。
1真菌孢子悬浮生长在3天10毫升的营养葡萄糖琼脂（毫升）被彻底融化混合20毫升沙氏琼脂并灭菌培养皿倒。当琼脂成立，5毫米直径的6对种子每盘了水井。这些洞充满了100毫升的试验在
g样品。所有这些实验，一式两份。孵育的Petri板在28 ° C的7-8天。所有被检查的培养板后24-96小时，结果表列。该区域的抑制植物组分产生了与标准（生产区相比，制霉菌素和灰黄霉素20毫克/毫升）。

Answer 3

该透析样本受一经Sephadex G - 25柱和0.2米醋酸洗脱。每个部位的4.5毫升搜集有关馏分收集在48毫升/小时流量

在280 nm处测定吸光度，监察色谱分离蛋白。水的提取蛋白质含量测定的劳里等人的方法。 （1951年），并以此作为标准的牛血清白蛋白。

汇集组分均匀性进行了检查用SDS凝胶电泳采用光盘对变性凝胶样品表面50毫升。电力供应在5毫安调整为80分钟，每管电流。凝胶与考马斯亮蓝R 250染色和destained与甲醇：乙酸：水由戴维斯报道，阿梅斯和里克伍德。

培养中生长的细菌在37℃过夜进行检测，从对经Sephadex G - 25分离不同组分的抗菌活性和水提取物对一oblongifolia用树叶。该抗菌活性检查种子平板法所报道拉希德等..这种技术肉类提取物营养琼脂培养基中的1.5％调整为pH值7.0，分布在40毫升瓶螺杆上限数量和消毒。细菌培养，然后加入到无菌的琼脂在45℃中，混合好，倒在消毒的Petri板立即。硬化后，直径6毫米以及切成琼脂和100毫升的一oblongifolia离开提取和分数在这些油井上。板块孵育37 ° C和意见，是经过24-72小时该区域的抑制植物组分产生了比较抗生素与商业标准，如Tarvid和Enaxbid光盘生产区的抑制作用。

抗真菌活性，对黑曲霉A25717测试，烟曲霉A9381，甲fla6us A15197和青霉海事39761。扩散板的方法来测试一oblongifolia留下轻微的修改分数由拉斯等报道。 （1995年）。这种技术，0.1毫升的真菌孢子suspensi

Answer 4

该透析样本受一经Sephadex G - 25柱和0.2米醋酸洗脱。每个部位的4.5毫升搜集有关馏分收集在48毫升/小时流量

在280 nm处测定吸光度，监察色谱分离蛋白。水的提取蛋白质含量测定的劳里等人的方法。 （1951年），并以此作为标准的牛血清白蛋白。

汇集组分均匀性进行了检查用SDS凝胶电泳采用光盘对变性凝胶样品表面50毫升。电力供应在5毫安调整为80分钟，每管电流。凝胶与考马斯亮蓝R 250染色和destained与甲醇：乙酸：水由戴维斯报道，阿梅斯和里克伍德。

培养中生长的细菌在37℃过夜进行检测，从对经Sephadex G - 25分离不同组分的抗菌活性和水提取物对一oblongifolia用树叶。该抗菌活性检查种子p