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Question

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马链球菌(Streptococcus equi)属于兰氏分菌的C群链球菌，包括马链球菌马亚种(Streptococcus. equi subsp. equi)、马链球菌兽疫亚种(S. equi subsp. zooepidemicus)和马链球菌类马亚种(S. equi subsp. equisi milis).该菌可通过外伤、手术、或治疗等途径感染多种动物,引发败血症Sepsis、脑膜炎Encephalitis、乳腺炎(Mastitis)等疾病.其特定基因表达产物对细菌致病性(Pathogenicity)有着特殊意义.
研究基因，就是为了要了解其功能和表达及调控方式。利用生物信息学(Bioinformatics)可以对基因的功能和表达调控进行预测和分析，省去了烦琐的实验探索，为实验验证提供指导方向。基因的功能和序列是密切相关的，当序列的相似性超过一定范围时，它们可能执行相同的功能，通过将未知功能序列和已知功能序列的对比，如果它们的相似性较高就可以推断序列的相应功能。
本课题研究的目的就是以生物信息学作为理论基础运用工具软件和基于WEB的在线服务对某一未知马链球菌基因进行一系列的分析,结果整合,从而预测这一基因表达产物的功能.
马链球菌P50基因序列长度为100BP,整个基因有一个开放阅读框(ORF)全长90BP,编码100个氨基酸残基.
首先进入NCBI提供的BLAST服务网业,将处理过的马链球菌P50基因序列填入网业上的表单上,选择相应的参数提交到数据服务器上进行同源性检索(homology Search) .许多生物信息中心都有专门运行的BLAST服务器.这里应用http:www. ncbi.nlm.nih.govˆb lastˆ(运行BLASTR2.0,美国,维护GenBank)
然后根据BLAST检索(Search)的结果发现与马链球菌P50基因序列表达的蛋白质同源性最高的3种蛋白质依次为 123
其次应用DNASTAR软件将马链球菌P50基因序列翻译成氨基酸序列,结果显示P50基因序列共翻译20个氨基酸.
将与马链球菌P50氨基酸序列同源性高的序列分别输入到工具软件中，进行进化树(Phylogenetic Tree)的绘制。
将马链球菌P50氨基酸(Amino Acids)序列(Sequence)提交到专业网站中，即可预测该氨基酸序列的二级结构.然后利用软件分析P50基因蛋白质理化性质.
达尔文的进化论(Evolutionary theory) 给比较基因组学(comparative genomics)提供了理论依据。亲缘关系(kinship)越近，其基因序列的同源性(homology)就越高.两个序列的同源性越高,那么很可能表达产物(Expression Product)的功能就越相似.经过对结果的整理.分析,根据发现马链球菌P50基因表达的可能是一种底物结合的脂蛋白(Substrate binding Lipoprotein),直接参与金属转运系统(Transport System)的组成,该种转运系统在细菌从宿主(HOST) 体内获得营养的过程中起着至关重要的作用.这种蛋白是一个运输如铁、锰、锌等金属离子的底物结合的脂蛋白(Substrate binding Lipoprotein),我们可以称之为链球菌金属结合脂蛋白(Metal binding lipoproteins ,MBLS).这种蛋白作为马链球菌(Streptococcus equi)的一种毒力因子(virulent factor)是细菌致病的基础对马链球菌生理生化特性及致病性有着十分重要的影响和意义.

Answer 1

The horse streptococci(the Streptococcus equi) belong to the 兰 surname germ for cent of the Cs streptococci, include the horse streptococci MA3 YA3 ZHONG3 (Streptococcus. equi subsp. Equi),the horse streptococci monster epidemic disease second kind(S. equi subsp. Zooepidemicus)with horse streptococci MA3 YA3 ZHONG3 (S. equi subsp. equisi milis).That germ can pass external injury,surgical operation,or cure etc. the path infect various animals, causing diseases, such as septicemia Sepsis,meningitis Encephalitis and mastitis(Mastitis)...etc..Its particular gene expression outcome is pathogenic(Pathogenicity) to germ to have special meaning.
Study gene, be in order to understand its function and expression and adjust to control a way.The information of make use of the living creature's learn(Bioinformatics) can adjust to control to carry on the estimate and analysis to the function and expression of the gene, the province goes to a trivial experiment to investigate, providing the instruction direction for the experiment verification.The function and sequence of the gene are closely-related, when the likeness that is a sequence exceeds certain scope, they carry out same function probably, pass will don't know the function sequence with have already known the contrast of the function sequence, if their likeness is higher and then can predict a sequence to correspond function.
The purpose of this topic research is to be learn with the living creature information Be the theories foundation usage tool software with according to WEB on-line service to some a do not know the horse streptococci gene carry on a series of analysis, the result integrate, predicting the function of this gene expression outcome thus.
The horse streptococci P50 gene sequence length is a 100 BPs, the whole gene has a to open all of the reading frame(ORF)s to grow a 90 BPs, coding 100 amino acids 残基s.
The horse streptococci P50 the gene sequence entered the BLAST service net industry that the NCBI provide first, handle stuffs with the form and list of the net industry up, the choice correspond of the parameter hand over to the data server to go forward to act like the source index(the homology Search).Many living creature information centers all have the BLAST server of the specialized movement.Here applied http:www. ncbi.nlm.nih.gov?b last?(Circulate BLASTR2.0, the United States, the maintenance GenBank)
Then the result detection of the basis BLAST index(Search) and the protein of the horse streptococci P50 gene sequence expression together the source is the tallest of 3 kinds of protein one by one in order is 123
The secondly applied DNASTAR software translates the horse streptococci P50 gene sequence into the amino acids sequence, the result manifestation P50 gene sequence translates 20 amino acidses totally.
Will and the horse streptococci P50 amino acids sequence together source the high sequence inputs to the tool software respectively, carrying on evolving a tree(the Phylogenetic Tree) to draw.
Hand over the horse streptococci P50 amino acidses(the Amino Acids) sequence(Sequence) to the professional website in, can immediately predict the second class structure of that amino acids sequence.Then make use of a software analysis P50 gene protein physics and chemistry property.
The Darwinian Theory of Evolution(the Evolutionary theory) gave comparison the gene set to learn to provide the theories basis.The relative relation(kinship) is more near, its gene sequence is a source(homology) together more high.Two sequences together the source is more high, may express the function of the outcome(the Expression Product) very much so more alike.Through the sorting to the result.Analytical, according to discover the horse streptococci P50 genes express of may be the fat egg white(the Substrate binding Lipoprotein) that a kind of bottom thing combine, participate the metals transport system(the Transport System) to constitute directly, the kind's transport system has in the germ from process acquire nourishment inside the host(HOST) body to close an important function.This kind of egg white is the fat egg white(the Substrate binding Lipoprotein) that a conveyance,such as the bottom thing of the metals ions, such as iron,manganese and zinc...etc., combine, we can call it as the streptococci metals to combine the fat egg white.(the Metal binding lipoproteins, MBLS)This kind of egg white is a kind of poisonous dint factor(the virulent factor) of the horse streptococci(the Streptococcus equi) is a germ with the result that the foundation of the disease to the horse streptococci physiology bio-chemical characteristic and pathogenic have very important influence and the meaning.
回答者：6392541196 - 同进士出身 六级 3-9 20:52

The horse streptococci(Streptococcus equi) belongs to an orchid surname the Cs of the germ a streptococci for cent and include the horse streptococci Ma3 Ya3 's kind(Streptococcus. equi subsp. Equi), the horse streptococci monster epidemic disease second kind(S. equi subsp. Zooepidemicus)with the horse Lian4 Qiu2 Jun4 Lei4 Ma3 Ya3 's kind(S. equi subsp. equisi milis).That germ can pass external injury, surgical operation, or cure etc. the path infect various animals and cause diseases, such as septicemia Sepsis, meningitis Encephalitis and mastitis(Mastitis)...etc..It particular gene expression the outcome be pathogenic(Pathogenicity) to germ to have special meaning.
Study gene, be in order to understand its function and expression and adjust to control a way.The information of make use of the living creature learn(Bioinformatics) and can adjust to control to carry on an estimate and analyze to function and expression of gene, saved to go to a trivial experiment to investigate, for test a verification to provide an instruction direction.Function and sequence of gene are closely-related, the likeness which is a sequence exceed certain scope, they probably carry out same function and pass will don't know a function sequence and have already known the contrast of function sequence, if their likeness is higher and then can predict a correspond of sequence function.
The purpose of this topic research be is learn with living creature information Be the usage tool of the theories foundation software with according to WEB of on-line service to some a have never known a horse streptococci the gene carries on a series of analysis and the result integrate and thus predict that this gene expresses the function of outcome.
The gene sequence of the horse streptococci P50 length is a 100 BPs and the whole gene has 1 to open all of the reading frame(ORF)s to grow a 90 BPs and code 100 amino acids remnants Ji.
The gene sequence of the horse streptococci P50 got into the BLAST service net industry that the NCBI provide first, once handle stuffs with the form and list on the net industry up, the choice correspond of the parameter hand over a data server to go forward to act like a source an index(homology Search).The many living creature information center all has the BLAST server of specialized movement.Here applied http:www. ncbi.nlm.nih.gov?b last?(Circulate BLASTR2.0, the United States, support GenBank)
Then the protein of the search result detection of the basis BLAST and the gene sequence of the horse streptococci P50 expression together the source be the tallest of 3 kinds of proteins one by one in order is 123
The secondly applied DNASTAR software translates the gene sequence of the horse streptococci P50 into an amino acids sequence and the gene sequence of the result manifestation P50 totally translates 20 amino acidses.
Will with the amino acids sequence of the horse streptococci P50 together source the sequence of the Gao inputs tool software respectively and carry on evolving a draw of tree(Phylogenetic Tree).
Hand over the amino acidses(Amino Acids) sequence(Sequence) of the horse streptococci P50 to the professional website in, then predict the second class structure of that amino acids sequence.Then make use of the physics and chemistry property of the gene protein of the software analysis P50.
The Darwinian Theory of Evolution(Evolutionary theory) give comparison gene the set learn to provide a theories basis.The relative relation(kinship) is more near, its gene sequence together source(homology) more high.Two sequences together the source be more high, so and very may express the function of outcome(Expression Product) more alike.The process i

Answer 2

The horse streptococci(the Streptococcus equi) belong to the 兰 surname germ for cent of the Cs streptococci, include the horse streptococci MA3 YA3 ZHONG3 (Streptococcus. equi subsp. Equi),the horse streptococci monster epidemic disease second kind(S. equi subsp. Zooepidemicus)with horse streptococci MA3 YA3 ZHONG3 (S. equi subsp. equisi milis).That germ can pass external injury,surgical operation,or cure etc. the path infect various animals, causing diseases, such as septicemia Sepsis,meningitis Encephalitis and mastitis(Mastitis)...etc..Its particular gene expression outcome is pathogenic(Pathogenicity) to germ to have special meaning.
Study gene, be in order to understand its function and expression and adjust to control a way.The information of make use of the living creature's learn(Bioinformatics) can adjust to control to carry on the estimate and analysis to the function and expression of the gene, the province goes to a trivial experiment to investigate, providing the instruction direction for the experiment verification.The function and sequence of the gene are closely-related, when the likeness that is a sequence exceeds certain scope, they carry out same function probably, pass will don't know the function sequence with have already known the contrast of the function sequence, if their likeness is higher and then can predict a sequence to correspond function.
The purpose of this topic research is to be learn with the living creature information Be the theories foundation usage tool software with according to WEB on-line service to some a do not know the horse streptococci gene carry on a series of analysis, the result integrate, predicting the function of this gene expression outcome thus.
The horse streptococci P50 gene sequence length is a 100 BPs, the whole gene has a to open all of the reading frame(ORF)s to grow a 90 BPs, coding 100 amino acids 残基s.
The horse streptococci P50 the gene sequence entered the BLAST service net industry that the NCBI provide first, handle stuffs with the form and list of the net industry up, the choice correspond of the parameter hand over to the data server to go forward to act like the source index(the homology Search).Many living creature information centers all have the BLAST server of the specialized movement.Here applied http:www. ncbi.nlm.nih.gov?b last?(Circulate BLASTR2.0, the United States, the maintenance GenBank)
Then the result detection of the basis BLAST index(Search) and the protein of the horse streptococci P50 gene sequence expression together the source is the tallest of 3 kinds of protein one by one in order is 123
The secondly applied DNASTAR software translates the horse streptococci P50 gene sequence into the amino acids sequence, the result manifestation P50 gene sequence translates 20 amino acidses totally.
Will and the horse streptococci P50 amino acids sequence together source the high sequence inputs to the tool software respectively, carrying on evolving a tree(the Phylogenetic Tree) to draw.
Hand over the horse streptococci P50 amino acidses(the Amino Acids) sequence(Sequence) to the professional website in, can immediately predict the second class structure of that amino acids sequence.Then make use of a software analysis P50 gene protein physics and chemistry property.
The Darwinian Theory of Evolution(the Evolutionary theory) gave comparison the gene set to learn to provide the theories basis.The relative relation(kinship) is more near, its gene sequence is a source(homology) together more high.Two sequences together the source is more high, may express the function of the outcome(the Expression Product) very much so more alike.Through the sorting to the result.Analytical, according to discover the horse streptococci P50 genes express of may be the fat egg white(the Substrate binding Lipoprotein) that a kind of bottom thing combine, participate the metals transport system(the Transport System) to constitute directly, the kind's transport system has in the germ from process acquire nourishment inside the host(HOST) body to close an important function.This kind of egg white is the fat egg white(the Substrate binding Lipoprotein) that a conveyance,such as the bottom thing of the metals ions, such as iron,manganese and zinc...etc., combine, we can call it as the streptococci metals to combine the fat egg white.(the Metal binding lipoproteins, MBLS)This kind of egg white is a kind of poisonous dint factor(the virulent factor) of the horse streptococci(the Streptococcus equi) is a germ with the result that the foundation of the disease to the horse streptococci physiology bio-chemical characteristic and pathogenic have very important influence and the meaning.

Answer 3

The horse chain coccus (Streptococcus equi) belongs to a blue minutefungus C crowd of chain coccuses, including horse chain coccus horsesubspecies (Streptococcus. equi subsp. equi), horse chain coccus兽疫 subspecies (S. equi subsp. zooepidemicus) and horse hammerfungus category horse subspecies (S. equi subsp. equisi milis) Thisfungus passable flesh wound, surgery, or way infection many kinds ofanimals and so on treatment, initiation septicaemia Sepsis, meningitisEncephalitis, mastitis (Mastitis) and so on disease Its specific geneexpresses the product (Pathogenicity) to have the special significanceto the bacterium pathogenicity The research gene, is for must understand its function and theexpression and the regulative way. (Bioinformatics) may carries on theforecast and the analysis using the biological information study tothe gene function and the expression regulation, has omitted thetroublesome experimental exploration, confirms for the experimentprovides the instruction direction. The gene function and the sequenceare the close correlation, when the sequence similarity surpasses thecertain scope, they possibly carry out the same function, throughunknown function sequence and known function sequence contrast, iftheir similarity comparatively takes a higher position may infer thesequence the corresponding function. This topic research goal is carries on a series of analyses by thebiological information study as the rationale using tool software andbased on the WEB on-line service to some unknown horse chain coccusgene, finally conformity, thus forecast this gene expresses theproduct the function The horse chain coccus P50 gene sequence length is 100BP, the entiregene has an opening reading frame (ORF) span 90BP, codes 100 aminoacids remnant bases First enters the BLAST service network industry which NCBI provides,has processed the horse chain coccus P50 gene sequence fills in netindustry in the table list, chooses the corresponding parameter tosubmit to the data server on carries on the homology retrieval(homology Search) Many living thing information center all has thespecially movement the BLAST server Here applies http:www.ncbi.nlm.nih.govb last (to move the BLASTR2.0, US, maintains GenBank) Then acts according to the BLAST retrieval (Search) the resultdiscovery and the horse chain coccus P50 gene sequence expressionprotein homology highest 3 breeding eggs white matter is 123 in turn Next translates using DNASTAR software the horse chain coccusP50 gene sequence the amino acid sequence, finally demonstrated theP50 gene sequence altogether translates 20 amino acids Separately will input with the horse chain coccus P50 amino acidsequence homology high sequence to tool software in, will carry on theevolution tree (Phylogenetic Tree) plan. (Amino Acids) the sequence (Sequence) submits the horse chain coccusP50 amino acid to the specialized website in, then forecasts thisamino acid sequence two levels of structures Then analyzes the P50gene protein physics and chemistry nature using software Darwin's theory of evolution (Evolutionary theory) (comparativegenomics) has provided the theory basis to the comparison gene groupstudy. Blood relationship relations (kinship) nearer, its genesequence homology (homology) is higher Two sequences homology higher,then very possibly expresses the product (Expression Product) thefunction is more similar Passes through to the result reorganizationThe analysis, according to discovered the horse chain coccus P50 geneexpression possibly is one kind of substrate union lipoprotein(Substrate binding Lipoprotein), directly participates in the metaltransportation system (Transport System) the composition, this kind oftransportation system (HOST) in vivo obtains the nutrition in thebacterium from the host in the process the very important functionThis kind of protein is metallic ion the and so on a transportationlike iron, manganese, zinc substrate union lipoprotein (Substratebinding Lipoprotein), we may call it the chain coccus metal unionlipoprotein (Metal binding lipoproteins, MBLS) This kind of proteinas horse chain coccus (Streptococcus equi) one kind of toxicity factor(virulent factor) is the bacterium pathogenesis foundation has theextremely important influence and the significance to the horse chaincoccus physiology biochemistry characteristic and the pathogenicity

Answer 4

The horse streptococci(Streptococcus equi) belongs to an orchid surname the Cs of the germ a streptococci for cent and include the horse streptococci Ma3 Ya3 's kind(Streptococcus. equi subsp. Equi), the horse streptococci monster epidemic disease second kind(S. equi subsp. Zooepidemicus)with the horse Lian4 Qiu2 Jun4 Lei4 Ma3 Ya3 's kind(S. equi subsp. equisi milis).That germ can pass external injury, surgical operation, or cure etc. the path infect various animals and cause diseases, such as septicemia Sepsis, meningitis Encephalitis and mastitis(Mastitis)...etc..It particular gene expression the outcome be pathogenic(Pathogenicity) to germ to have special meaning.
Study gene, be in order to understand its function and expression and adjust to control a way.The information of make use of the living creature learn(Bioinformatics) and can adjust to control to carry on an estimate and analyze to function and expression of gene, saved to go to a trivial experiment to investigate, for test a verification to provide an instruction direction.Function and sequence of gene are closely-related, the likeness which is a sequence exceed certain scope, they probably carry out same function and pass will don't know a function sequence and have already known the contrast of function sequence, if their likeness is higher and then can predict a correspond of sequence function.
The purpose of this topic research be is learn with living creature information Be the usage tool of the theories foundation software with according to WEB of on-line service to some a have never known a horse streptococci the gene carries on a series of analysis and the result integrate and thus predict that this gene expresses the function of outcome.
The gene sequence of the horse streptococci P50 length is a 100 BPs and the whole gene has 1 to open all of the reading frame(ORF)s to grow a 90 BPs and code 100 amino acids remnants Ji.
The gene sequence of the horse streptococci P50 got into the BLAST service net industry that the NCBI provide first, once handle stuffs with the form and list on the net industry up, the choice correspond of the parameter hand over a data server to go forward to act like a source an index(homology Search).The many living creature information center all has the BLAST server of specialized movement.Here applied http:www. ncbi.nlm.nih.gov?b last?(Circulate BLASTR2.0, the United States, support GenBank)
Then the protein of the search result detection of the basis BLAST and the gene sequence of the horse streptococci P50 expression together the source be the tallest of 3 kinds of proteins one by one in order is 123
The secondly applied DNASTAR software translates the gene sequence of the horse streptococci P50 into an amino acids sequence and the gene sequence of the result manifestation P50 totally translates 20 amino acidses.
Will with the amino acids sequence of the horse streptococci P50 together source the sequence of the Gao inputs tool software respectively and carry on evolving a draw of tree(Phylogenetic Tree).
Hand over the amino acidses(Amino Acids) sequence(Sequence) of the horse streptococci P50 to the professional website in, then predict the second class structure of that amino acids sequence.Then make use of the physics and chemistry property of the gene protein of the software analysis P50.
The Darwinian Theory of Evolution(Evolutionary theory) give comparison gene the set learn to provide a theories basis.The relative relation(kinship) is more near, its gene sequence together source(homology) more high.Two sequences together the source be more high, so and very may express the function of outcome(Expression Product) more alike.The process is to the tidy up of result.Analytical, according to discover the horse streptococci P50 the genes express of may be the fat egg white(the Lipoprotein of the Substrate binding) that a kind of bottom thing combine, directly participate the constitute of system(Transport System) of the metals transport, that kind of transport system acquires to have in the nutrient process in the germ from the host(HOST) body to close an important function.This kind of egg white is the fat egg white(the Lipoprotein of the Substrate binding) that a conveyance,such as the bottom thing of metals ions, such as iron, manganese and zinc...etc., combine, we can call it as the streptococci metals combines fat egg white.(the lipoproteins of the Metal binding, MBLS)This kind of egg white's ising a kind of poisonous dint factor(virulent factor) of horse streptococci(Streptococcus equi) is a germ with the result that the foundation of disease to the physiology bio-chemical characteristic of the horse streptococci and pathogenic have very important influence and meaning