麻烦各位大虾帮忙翻译一下下面的文字成英文，万分感谢！！！
1. 左手固定小鼠，使鼠的腹部面向捉持者，鼠头略朝下。右手持注射器从一侧下腹部进行穿刺，注射针与皮肤面呈45度刺入腹肌，针头刺入皮肤后进针3－5mm左右，当感到落空感时表示已进入腹腔，回抽无血液、肠液、尿液后即可注射。注射量0.1~0.2ml/10g体重。本实验用生理盐水练习操作。
2. 捉拿固定好小鼠后（方法同上），右手持灌胃器经口角将灌胃管插入口腔。用灌胃管轻压小鼠上腭部，使口腔和食管成一直线，再将灌胃管沿上腭缓缓插入食管，进针2－3cm左右即可注药（注意）。
灌注量为0.1~0.3ml/10g体重。本实验用5％炭粉混悬液进行灌胃操作，是否灌胃成功可解剖观察。
3. 手法正确，进针很顺且动物呼吸无异常；
如进针遇到阻力，应退出重来，不能强插。以免刺破食管或误入气管，使动物死亡。
4. 注射时，先将小鼠固定于固定器内(可采用筒底有小口的玻璃筒、金属或铁丝笼),将全部尾巴露出外面，使尾静脉充盈明显后，以左手拇指与食指捏住尾部两侧，右手持4号针头注射器，呈30度刺入静脉，注射成功后，拔出针头，轻按注射部位止血。一次注射量0.05-0.1 ml/10g。
5. 推注药液无阻力，且可见沿静脉血管出现一条白线，说明针头在血管内，可继续注药。
如遇到阻力较大，皮下发白且有隆起时，说明针头不在静脉内，须拔出针头重新穿刺。
6. 用手指轻弹尾尖部
必要时可用45－50℃的温水浸泡尾部
用75%酒精或二甲苯涂擦尾部
或将小鼠放于40－50℃的加热板作运动
通过以上方法，就可使小鼠全部血管扩张充血、表皮角质软化，易于穿刺。一般选择尾两侧静脉，并宜从尾尖端开始，渐向尾根部移动，以备反复应用。
7.昆明种小鼠：
1946年从印度引入昆明，体型小;产仔多，生长繁殖快，饲养管理方便;对环境的适应性和对疾病的抵抗力强;被广泛用于药理、毒理、病毒、微生物的研究以及生物制品、药品的鉴定，是常用的实验动物。
谢绝弄个在线翻译过来，希望能够有好心的大虾给出地道的翻译，因为是要授课，不甚感激！！！
Fixed mouse left-handed, so to catch mice in the stomach for the supporters, mouse head slightly down. Syringe from the right hand side of the lower abdomen to puncture, the needle piercing the skin and abdominal muscles appear before 45 degrees, the needle piercing the skin of about 3-5mm needle backward, When you feel a sense of frustration that has entered the abdominal cavity, Withdrawing without blood, intestinal fluid, urine, immediately after injection. Injection volume of 0.1 ~ 0.2ml/10g weight. In this study, to practice operations with normal saline.
2. To catch mice and fixed (as above), right hand numbness of the mouth will be fed by gavage tube device into the mouth. Gently press with a stomach tube on the palate in mice, so that the mouth and esophagus in a straight line, and then slowly fed into the tube along the esophagus on the palate, about 2-3cm needle injection can be (note).
Infusion volume of 0.1 ~ 0.3ml/10g weight. In this study, carried out with 5% charcoal suspension stomach operation, it can be fed successfully anatomical observation.
3. Means the right, the needle is smooth and no abnormal breathing animals;
Encounter resistance as the needle should be out again, not strong interpolation. So as not to puncture the esophagus or strayed into the trachea, the animals died.
4. Injection, mice were first fixed in a fixed inside (the end of a small population can be tube glass tube, metal or wire cage), exposed to all the tail out so clearly after tail vein filling to the left thumb and index finger pinching live on both sides of the tail, right hand holding the syringe needle 4, piercing the vein was 30 degrees, injection is successful, pull out the needle, touch the injection site to stop bleeding. An injection volume of 0.05-0.1 ml/10g.
5. Bolus injection of liquid without resistance, and can be seen along the veins of a white line appears, indicating that needle in the blood vessels can continue to inject drugs.
In case of resistance to large, pale skin and a raised, indicating the intravenous needle is not required to pull out the needle to re-puncture.
6. End of tail flick with your fingers
Necessary, can be soaked in warm water tail of 45-50 ℃
With 75% alcohol or xylene rubbed tail
Or the mice placed on a hot plate for 40-50 ℃ Sports
Through the above method, you can make all the vascular dilatation and congestion in mice, skin softening skin, easy to puncture. General choice of the end of both sides of the vein, and should start from the tail tip, moving gradually to the tail root to prepare for repeated applications.
7. Kunming mice:
Introduced from India in 1946, Kunming, size small; calving and more rapid growth and reproduction, breeding management, and convenient; on the environment adaptability and strong resistance to disease; is widely used in pharmacology, toxicology, viral, microbial studies and biological products, drugs identification, is commonly used in experimental animals.
2. To catch mice and fixed (as above), right hand numbness of the mouth will be fed by gavage tube device into the mouth. Gently press with a stomach tube on the palate in mice, so that the mouth and esophagus in a straight line, and then slowly fed into the tube along the esophagus on the palate, about 2-3cm needle injection can be (note).
Infusion volume of 0.1 ~ 0.3ml/10g weight. In this study, carried out with 5% charcoal suspension stomach operation, it can be fed successfully anatomical observation.
3. Means the right, the needle is smooth and no abnormal breathing animals;
Encounter resistance as the needle should be out again, not strong interpolation. So as not to puncture the esophagus or strayed into the trachea, the animals died.
4. Injection, mice were first fixed in a fixed inside (the end of a small population can be tube glass tube, metal or wire cage), exposed to all the tail out so clearly after tail vein filling to the left thumb and index finger pinching live on both sides of the tail, right hand holding the syringe needle 4, piercing the vein was 30 degrees, injection is successful, pull out the needle, touch the injection site to stop bleeding. An injection volume of 0.05-0.1 ml/10g.
5. Bolus injection of liquid without resistance, and can be seen along the veins of a white line appears, indicating that needle in the blood vessels can continue to inject drugs.
In case of resistance to large, pale skin and a raised, indicating the intravenous needle is not required to pull out the needle to re-puncture.
6. End of tail flick with your fingers
Necessary, can be soaked in warm water tail of 45-50 ℃
With 75% alcohol or xylene rubbed tail
Or the mice placed on a hot plate for 40-50 ℃ Sports
Through the above method, you can make all the vascular dilatation and congestion in mice, skin softening skin, easy to puncture. General choice of the end of both sides of the vein, and should start from the tail tip, moving gradually to the tail root to prepare for repeated applications.
7. Kunming mice:
Introduced from India in 1946, Kunming, size small; calving and more rapid growth and reproduction, breeding management, and convenient; on the environment adaptability and strong resistance to disease; is widely used in pharmacology, toxicology, viral, microbial studies and biological products, drugs identification, is commonly used in experimental animals.
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第1个回答 2011-03-09
1.Left hand, make the fixed mice rat catching hold of abdominal oriented, rat head slightly down. Right hand hold syringe from one side of the lower abdomen for puncture, inject needle and skin angled 45 degrees Pierce abdominal muscle, needle pierced skin lifo needle 3-5mm or so, as feel frustrated when feeling says has entered the abdominal cavity, no blood, intestinal juice to smoke, urine, can injections. Injection quantity 0.1 ~ 0.2 ml / 10g weight. This experiment with physiological saline practice operation.
2.Seize fixed the mice (methods), the right hand hold alexandrine irrigation by stomach device quarrel will fill the stomach tube into the mouth. Using irrigation gastric tube gently press mice jaws, make oral and esophagus into a straight line, then put the irrigation gastric tube insertion slowly along the roof esophagus, the needle into 2-3cm around can note medicine (note).
Perfusion measure for 0.1 ~ 0.3 ml / 10g weight. This experiment with 5% toner suspension gastric lavage fluid filling operation, whether stomach successful can anatomic observation.
3.Gimmick correctly, the needle into the very shun and animal breath; no abnormalities,
If the needle into meets resistance, should quit from the heavy, not strong insert. Lest punctured esophageal or errant tracheal, kill animals.
4.Inject, first mouse is fixed to the holder (can adopt cylinder bottom has small mouth glass tube, metal or TieSiLong), will be exposed outside, make whole tail end vein filled with obvious which, after left thumb and forefinger niezhu rear two side, right hand hold 4 needleless syringe, show 30 degrees Pierce vein, injection after the success, draw needles and lightly in injection site bleeding. An injection quantity 0.05-0.1 ml / 10g.
5.Push note solution is no resistance, and visible along the veins appear a white line, explain needle inside blood-vessel, can continue to note medicine.
Such as the resistance is larger, skin white and uplift issued, the needle, must not draw intravenous needle biopsy again.
6.With finger flip the tail tips
When necessary usable 45 -- 50 ℃ of warm water for the tail
With 75% ethanol or xylene inunction tail
Or will put in the 40-50 mice ℃ heating plate movement
Through the above method, can make the mice all vascular dilatation and congestion, epidermis cutin bate, easy to puncture. Generally choose the tail, and appropriate from both sides vein tail tips start, gradually moving towards the tail root, ready to repeated applications.
7.Kunming mice:.
1946 brought from India kunming installs, volume is small, Breed more, to grow fast, raising management convenient; Adaptability to the environment of and resistance to illness; strong Widely used in pharmacology and toxicology, virus, microbe study and biological products, pharmaceuticals, are used in the identification of experimental animals.
2.Seize fixed the mice (methods), the right hand hold alexandrine irrigation by stomach device quarrel will fill the stomach tube into the mouth. Using irrigation gastric tube gently press mice jaws, make oral and esophagus into a straight line, then put the irrigation gastric tube insertion slowly along the roof esophagus, the needle into 2-3cm around can note medicine (note).
Perfusion measure for 0.1 ~ 0.3 ml / 10g weight. This experiment with 5% toner suspension gastric lavage fluid filling operation, whether stomach successful can anatomic observation.
3.Gimmick correctly, the needle into the very shun and animal breath; no abnormalities,
If the needle into meets resistance, should quit from the heavy, not strong insert. Lest punctured esophageal or errant tracheal, kill animals.
4.Inject, first mouse is fixed to the holder (can adopt cylinder bottom has small mouth glass tube, metal or TieSiLong), will be exposed outside, make whole tail end vein filled with obvious which, after left thumb and forefinger niezhu rear two side, right hand hold 4 needleless syringe, show 30 degrees Pierce vein, injection after the success, draw needles and lightly in injection site bleeding. An injection quantity 0.05-0.1 ml / 10g.
5.Push note solution is no resistance, and visible along the veins appear a white line, explain needle inside blood-vessel, can continue to note medicine.
Such as the resistance is larger, skin white and uplift issued, the needle, must not draw intravenous needle biopsy again.
6.With finger flip the tail tips
When necessary usable 45 -- 50 ℃ of warm water for the tail
With 75% ethanol or xylene inunction tail
Or will put in the 40-50 mice ℃ heating plate movement
Through the above method, can make the mice all vascular dilatation and congestion, epidermis cutin bate, easy to puncture. Generally choose the tail, and appropriate from both sides vein tail tips start, gradually moving towards the tail root, ready to repeated applications.
7.Kunming mice:.
1946 brought from India kunming installs, volume is small, Breed more, to grow fast, raising management convenient; Adaptability to the environment of and resistance to illness; strong Widely used in pharmacology and toxicology, virus, microbe study and biological products, pharmaceuticals, are used in the identification of experimental animals.
第2个回答 2011-03-09
1.Fixed mouse left-handed, so to catch mice in the stomach for the supporters, mouse head slightly down. Syringe from the right hand side of the lower abdomen to puncture, the needle piercing the skin and abdominal muscles appear before 45 degrees, the needle piercing the skin of about 3-5mm needle backward, When you feel a sense of frustration that has entered the abdominal cavity, Withdrawing without blood, intestinal fluid, urine, immediately after injection. Injection volume of 0.1 ~ 0.2ml/10g weight. In this study, to practice operations with normal saline.
2.After the capture of mice fixed (as above), right hand holding device by gavage stomach tube into the mouth to mouth. Gently press with a stomach tube on the palate in mice, so that the mouth and esophagus in a straight line, and then slowly fed into the tube along the esophagus on the palate, about 2-3cm needle injection can be (note). Infusion volume of 0.1 ~ 0.3ml/10g weight. In this study, carried out with 5% charcoal suspension stomach operation, it can be fed successfully anatomical observation.
3.Approach is correct, the needle is smooth and no abnormal breathing animals; such as needle met with resistance, should be out again, not strong interpolation. So as not to puncture the esophagus or strayed into the trachea, the animals died.
4.Injection, mice were first fixed in a fixed inside (the end of a small population can be tube glass tube, metal or wire cage), exposed to all the tail out so clearly after tail vein filling to the end of the left thumb and forefinger on both sides, right hand holding the syringe needle 4, piercing the vein was 30 degrees, injection is successful, pull out the needle, touch the injection site to stop bleeding. An injection volume of 0.05-0.1 ml/10g.
5.Liquid injection without resistance, and visible veins occur along a white line, indicating that needle in the blood vessels can continue to inject drugs. In case of resistance to large, pale skin and a raised, indicating the intravenous needle is not required to pull out the needle to re-puncture.
6.End of tail flick with your fingers if necessary, and the tail immersed in warm water 45-50 ℃ with 75% alcohol or xylene rubbed the mice were placed in the rear or 40-50 ℃ heating plate for movement through the above method, you can make All vascular dilatation and congestion in mice, skin softening skin, easy to puncture. General choice of the end of both sides of the vein, and should start from the tail tip, moving gradually to the tail root to prepare for repeated applications.
7.Kunming mice: introduction from India in 1946, Kunming, size small; calving and more rapid growth and reproduction, breeding management, and convenient; on the environment adaptability and strong resistance to disease; is widely used in pharmacology, toxicology, viral , Microorganisms and the biological products, pharmaceutical identification, is commonly used in experimental animals.追问
2.After the capture of mice fixed (as above), right hand holding device by gavage stomach tube into the mouth to mouth. Gently press with a stomach tube on the palate in mice, so that the mouth and esophagus in a straight line, and then slowly fed into the tube along the esophagus on the palate, about 2-3cm needle injection can be (note). Infusion volume of 0.1 ~ 0.3ml/10g weight. In this study, carried out with 5% charcoal suspension stomach operation, it can be fed successfully anatomical observation.
3.Approach is correct, the needle is smooth and no abnormal breathing animals; such as needle met with resistance, should be out again, not strong interpolation. So as not to puncture the esophagus or strayed into the trachea, the animals died.
4.Injection, mice were first fixed in a fixed inside (the end of a small population can be tube glass tube, metal or wire cage), exposed to all the tail out so clearly after tail vein filling to the end of the left thumb and forefinger on both sides, right hand holding the syringe needle 4, piercing the vein was 30 degrees, injection is successful, pull out the needle, touch the injection site to stop bleeding. An injection volume of 0.05-0.1 ml/10g.
5.Liquid injection without resistance, and visible veins occur along a white line, indicating that needle in the blood vessels can continue to inject drugs. In case of resistance to large, pale skin and a raised, indicating the intravenous needle is not required to pull out the needle to re-puncture.
6.End of tail flick with your fingers if necessary, and the tail immersed in warm water 45-50 ℃ with 75% alcohol or xylene rubbed the mice were placed in the rear or 40-50 ℃ heating plate for movement through the above method, you can make All vascular dilatation and congestion in mice, skin softening skin, easy to puncture. General choice of the end of both sides of the vein, and should start from the tail tip, moving gradually to the tail root to prepare for repeated applications.
7.Kunming mice: introduction from India in 1946, Kunming, size small; calving and more rapid growth and reproduction, breeding management, and convenient; on the environment adaptability and strong resistance to disease; is widely used in pharmacology, toxicology, viral , Microorganisms and the biological products, pharmaceutical identification, is commonly used in experimental animals.追问
万分感谢你的回应，但是看着像在线翻译过来的，希望能够有好心的大虾给出地道的翻译，因为是要授课，不甚感激！！！
第3个回答 2011-03-09
1.Left hand, make the fixed mice rat catching hold of abdominal oriented, rat head slightly down. Right hand hold syringe from one side of the lower abdomen for puncture, inject needle and skin angled 45 degrees Pierce abdominal muscle, needle pierced skin lifo needle 3-5mm or so, as feel frustrated when feeling says has entered the abdominal cavity, no blood, intestinal juice to smoke, urine, can injections. Injection quantity 0.1 ~ 0.2 ml / 10g weight. This experiment with physiological saline practice operation.
2. Seize fixed the mice (methods), the right hand hold alexandrine irrigation by stomach device quarrel will fill the stomach tube into the mouth. Using irrigation gastric tube gently press mice jaws, make oral and esophagus into a straight line, then put the irrigation gastric tube insertion slowly along the roof esophagus, the needle into 2-3cm around can note medicine (note).
Perfusion measure for 0.1 ~ 0.3 ml / 10g weight. This experiment with 5% toner suspension gastric lavage fluid filling operation, whether stomach successful can anatomic observation.
3. Technique correctly, the needle into the very shun and animal breath; no abnormalities,
If the needle into meets resistance, should quit from the heavy, not strong insert. Lest punctured esophageal or errant tracheal, kill animals.
4. To inject, first mouse is fixed to the holder (can adopt cylinder bottom has small mouth glass tube, metal or TieSiLong), will be exposed outside, make whole tail end vein filled with obvious which, after left thumb and forefinger niezhu rear two side, right hand hold 4 needleless syringe, show 30 degrees Pierce vein, injection after the success, draw needles and lightly in injection site bleeding. An injection quantity 0.05-0.1 ml / 10g.
5. Push note solution is no resistance, and visible along the veins appear a white line, explain needle inside blood-vessel, can continue to note medicine.
Such as the resistance is larger, skin white and uplift issued, the needle, must not draw intravenous needle biopsy again.
6. Fingers flip the tail tips
When necessary usable 45 -- 50 ℃ of warm water for the tail
With 75% ethanol or xylene inunction tail
Or will put in the 40-50 mice ℃ heating plate movement
Through the above method, can make the mice all vascular dilatation and congestion, epidermis cutin bate, easy to puncture. Generally choose the tail, and appropriate from both sides vein tail tips start, gradually moving towards the tail root, ready to repeated applications.
Kunming mice: 7.
1946 brought from India kunming installs, volume is small, Breed more, to grow fast, raising management convenient; Adaptability to the environment of and resistance to illness; strong Widely used in pharmacology and toxicology, virus, microbe study and biological products, pharmaceuticals, are used in the identification of experimental animals.
2. Seize fixed the mice (methods), the right hand hold alexandrine irrigation by stomach device quarrel will fill the stomach tube into the mouth. Using irrigation gastric tube gently press mice jaws, make oral and esophagus into a straight line, then put the irrigation gastric tube insertion slowly along the roof esophagus, the needle into 2-3cm around can note medicine (note).
Perfusion measure for 0.1 ~ 0.3 ml / 10g weight. This experiment with 5% toner suspension gastric lavage fluid filling operation, whether stomach successful can anatomic observation.
3. Technique correctly, the needle into the very shun and animal breath; no abnormalities,
If the needle into meets resistance, should quit from the heavy, not strong insert. Lest punctured esophageal or errant tracheal, kill animals.
4. To inject, first mouse is fixed to the holder (can adopt cylinder bottom has small mouth glass tube, metal or TieSiLong), will be exposed outside, make whole tail end vein filled with obvious which, after left thumb and forefinger niezhu rear two side, right hand hold 4 needleless syringe, show 30 degrees Pierce vein, injection after the success, draw needles and lightly in injection site bleeding. An injection quantity 0.05-0.1 ml / 10g.
5. Push note solution is no resistance, and visible along the veins appear a white line, explain needle inside blood-vessel, can continue to note medicine.
Such as the resistance is larger, skin white and uplift issued, the needle, must not draw intravenous needle biopsy again.
6. Fingers flip the tail tips
When necessary usable 45 -- 50 ℃ of warm water for the tail
With 75% ethanol or xylene inunction tail
Or will put in the 40-50 mice ℃ heating plate movement
Through the above method, can make the mice all vascular dilatation and congestion, epidermis cutin bate, easy to puncture. Generally choose the tail, and appropriate from both sides vein tail tips start, gradually moving towards the tail root, ready to repeated applications.
Kunming mice: 7.
1946 brought from India kunming installs, volume is small, Breed more, to grow fast, raising management convenient; Adaptability to the environment of and resistance to illness; strong Widely used in pharmacology and toxicology, virus, microbe study and biological products, pharmaceuticals, are used in the identification of experimental animals.
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