纺织方面的翻译

Question

The F. oxysporum strain used was 07 SD from
ESALQ-USP Genetic and Molecular Biology Laboratory-
Piracicaba, S.P., Brazil. The fungal inoculum was prepared
in 2% malt extract and 0.5% yeast extract at 28 C in
Petri dishes.The liquid fungal growth was carried out
in the presence of 0.5% yeast extract at 28 C for 6
days.The biomass was filtrated and resuspended in sterile
water.The biosynthesis of silver nanoparticles was
carried out as following: approximately 10 g of F. oxysporum
biomass was taken in a conical flask containing
100 ml of distilled water, kept for 72 h at 28 C and
then the aqueous solution components were separated by
filtration.In this solution (fungal filtrate) AgNO3 (10−3
M) was added and the system was kept for several hours
at 28 C.Periodically , aliquots of the reaction solution
were removed and the absorption was measured in a
UV-Vis spectrophotometer (Agilent 8453—diode array) at
440 nm.The silver nanoparticles were characterized by
Transmission Electron Microscopy (TEM) and Elemental
Spectroscopy Imaging (ESI).Bright field images and
the elemental distribution within silver nanoparticles were
obtained using a Carl Zeiss CEM-902 transmission electron
microscope (80 KeV), equipped with a Castaing-
Henry-Ottensmeyer energy filter spectrometer within the
column.F or the examination of the silver particle, one
drop of the particle dispersion was deposited on carboncoated
parlodion films supported in 300 mesh copper grids
(Ted Pella).
Elemental images were obtained for the relevant elements
found in this sample, using monochromatic electrons
corresponding to the silver K-edge, sulfur L2
3-edge,
and nitrogen L3-edge.The energy-selecting slit was set at
367 ± 6 keV for Ag, 165 ± 6 eV for S, and 400 ± 6 eV
for N.The images were recorded by a Proscan high-speed
slow-scan CCD camera and processed in the AnalySis 3.0
system.
The size of silver nanoparticles was measured by X-Ray
Diffraction (XRD, model XD3A from Shimadzu) with
nickel-filtered Cu-K radiation (40 KV, 30 mA) at an
angle of 2 from 5 to 50.The scan speed was 0.02/min
204 J. Biomed. Nanotechnol. 3, 203–208, 2007
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Durán et al. Antibacterial Effect of Silver Nanoparticles Produced by Fungal Process on Textile Fabrics
and the time constant was 2 s.The size was calculated
through of the Scherrer’s equation:
D = K/
cor cos 
with
cor
= 
2
sample
−
2
ref 1/2
where D is the average crystal size, K is the Scherrer coefficient
(0.89),  is the X-ray wavelength ( = 1
542 Å), 
Bragg’s angle (2 = 251),
cor the corrected of the full
width at half-maximum (FWHM) in radians, and
sample
and
ref are the FWHM of the reference and sample peaks,
respectively.21

Answer 1

枯萎病菌菌株的用的是从政府统计处07
ESALQ -美国药典的遗传和分子生物学实验室，
皮拉西卡巴，S.P.，巴西。真菌接种的准备
2％的麦芽提取物和酵母提取物0.5％，28？C的
真菌生长的Petri dishes.The液进行
在0.5％的酵母提取物的存在，28 6摄氏度
days.The生物量过滤和无菌悬浮
银纳米粒子合成的water.The
进行了如下：约10克的F. oxysporum
生物质被包含在一个锥形烧瓶
100毫升的蒸馏水，保持在72小时28摄氏度和
那么水溶液成分分离
filtration.In这一解决方案（真菌滤液）硝酸银（10-3
M）在加入该系统是为几个小时不断
在28？长周期，等分试样溶液的反应
被拆除的吸收是有分寸
紫外可见分光光度计（安捷伦8453二极管阵列）在
440 nm.The银纳米粒子进行了表征
透射电子显微镜（TEM）和元素
光谱成像（ESI）的。明场图像和
银纳米粒子内元素分布
使用卡尔蔡司获得澳电- 902透射电镜
显微镜（80千电子伏），配备了卡斯坦-
亨利- Ottensmeyer过滤器内的能量光谱仪
column.F或银粒子，一次考试
粒子分散的下降沉积carboncoated
parlodion电影支持300目铜网
（特德佩拉）。
获得图像元素的相关内容
发现在此示例，使用单色电子
相应的银K -边，硫的L2？ 3边，
和氮的L3 - edge.The能源选择狭缝定为
367 ± 6银，165 ± 6 keV的布道和400 ± 6伏特
为N的图像录制了Proscan高速
慢扫描CCD相机和分析3.0处理
系统。
银纳米粒子尺寸测量的X射线
衍射仪（XRD，模式，从日本岛津XD3A）与
镍过滤铜K吗？辐射（40千伏，30毫安）在一
角2？从5？到50？。扫描速度为0.02？/分钟
204 j的生物医学。 Nanotechnol。 3，203-208，2007
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周四，2007年6月21日0时48分47秒
杜兰等。抗菌作用的纺织面料生产的银纳米粒子真菌过程
和时间常数为2 s.The大小计算
通过谢乐公的公式：
Ð =？K吗？？/？
COS的心病？？？同
心病
=？
2
样本
-
2
裁判？1 / 2
其中D是平均晶粒尺寸，K是舍莱尔系数
（0.89），？是X射线波长（？= 1？542 Å），？
布拉格的角度（2 = 25？1？）
肺心病纠正的全部
宽度的一半，最高弧度（FWHM）的，及
样本
和
参考是参考和样品峰半高宽，
respectively.21